The rider, believed to be a middle-aged man from the Nottingham area, was riding a red Triumph motorbike which was in collision with a van and a car.
The other vehicles involved were a blue Daf van driven by a North Hykeham man and black Toyota saloon owned by a Lincoln resident.
Neither of the drivers were injured.
Congestion rippled across the city following the collision which occurred at 11.25am on Friday causing both carriageways of the A46 between the Riseholme and Nettleham roundabouts to close.
Spokesman for Lincolnshire Police, James Newall, said:
"This incident took place when there would have been a fair amount of traffic on the roads. It is vital that we get information from as many witnesses as possible while it is still relatively fresh in their minds.The Lincolnshire Road Safety Partnership confirmed this was the seventh fatality on the county's roads so far in 2010 and the fifth accident causing injury on this stretch of the A46 in five years.
"The road was closed for a significant amount of time to enable our collision experts to carry out their analysis of the scene and for recovery teams to clear the stretch."